Abstract: In order to explore the effects of biochar on tobacco leaf production under the treatments of deep tillage and green manure, field experiments were conducted to analyze the effects of different biochar application rates and application methods on the agronomic traits, economic traits and chemical composition of tobacco leaves, soil nutrients and microbial quantity. The results showed that the application of biochar under these conditions could improve the agronomic trait indexes such as plant height, leaf number, stem circumference, leaf area, thus increase the yield and output value of tobacco leaves, as well as the proportion of high grade leaves and promote the harmony of chemical composition of tobacco leaves. Biochar could raise the contents of soil organic matter, dissolved organic carbon, total nitrogen, available phosphorus, and available potassium. Compared with the combined treatment of deep tillage and biochar, the treatment of green manure and biochar was more beneficial to the reproduction of soil bacteria and fungi. By comprehensive comparison, green manure combined with spreading biochar over fields at the rate of 4 500 kg/hm² or applying biochar in holes at the rate of 750 kg/hm² had better effects on increasing soil nutrients as well as improving the quality and yield of flue-cured tobacco in central Henan Province.