基于高通量测序的烟草甲基因组调查分析

Genome survey of Lasioderma serricorne based on high-throughput sequencing

摘要: 为更好破译烟草甲全基因组信息，基于Illumina高通量测序平台对烟草甲基因组进行了调查分析。预测了其基因组大小，同时分析了基因组的杂合度、重复序列、GC含量等基因组基本特征，并在此基础上对烟草甲基因组进行了初步组装。研究表明：①烟草甲预估基因组大小为242.25 Mb，杂合率为0.77%，重复序列比率为42.95%，GC含量为44.61%。②基因组组装后，得到418 693条scaffolds，拼接序列总长为211.10 Mb，N50为1 864 bp。通过基因组调查分析为后续的烟草甲全基因组测序提供了重要的理论依据。

Abstract: In order to decipher the whole genome of Lasioderma serricorne, a genome survey was performed by high-throughput sequencing technology of Illumina. The genome size of L. serricorne was predicted, and the features of genomic heterogeneity, repeats and GC content were analyzed. Furthermore, the preliminary genome assembly was conducted. The results showed that the genome size of L. serricorne was 242.25 Mb. The heterogeneity percentage, repeats and GC content were 0.77%, 42.95% and 44.61%, respectively. Additionally, 418 693 scaffolds with a total length of 211.10 Mb were obtained after assembling. The scaffold N50 was 1 864 bp. The genome survey provides an important theoretical basis for the subsequent whole genome sequencing of L. serricorne.