Abstract: To further study the genetic information and to promote the research on identification, phylogeny and population genetics of Lasioderma serricorne, the next-generation sequencing was used to acquire the complete mitochondrial genome of L. serricorne. The results showed that the mitochondrial genome of L. serricorne was 15 009 bp in length, which contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 translocation RNA genes and a control region. The structure and organization of the genome were similar with those of the stored-product pests from Coleoptera. The A+T content of the whole mitochondrial genome was 78.7%. Except for cox2 whose start codon was TTG, the start codons of protein-coding genes were typical ATN codon. The stop codons of cox3, nad5, nad4 were incomplete T, and the other genes used the typical TAA or TAG as the stop codons. The 22 tRNA of L. serricorne could fold into a clover structure, however tRNA^Asp, tRNA^Leu, tRNA^His and tRNA^ser(AGN) folded into atypical cloverleaf secondary structures with the absence of either TψC-arm or D-arm.