Abstract: To investigate the release behaviors of main components from heated cigarettes at different heating temperatures, aerosol collection mass (ACM) and three main aerosol components (nicotine, glycerol and moisture) were measured from five electrically heated cigarettes using a bespoke test platform by varying heating temperature within 250-375 ℃. The results showed that: 1) The ACMs were quite different from each other, as well as the releases of the three components; heating temperature was an important factor for their releases. 2) Around 350 ℃ was the turning point for the ACMs and the release behaviors of the three components for most cigarettes, the levels of ACMs, nicotine, glycerol and moisture reaching 15.55-26.51, 0.18-0.45, 0.37-1.37, 9.72-14.14 mg/cigarette respectively. The proportions of nicotine, glycerol and moisture in ACMs were 0.89%-1.85%, 1.53%-8.41%, 52.38%-62.53% respectively. The transfer rates of nicotine and glycerol from tobacco materials to ACMs were in the ranges of 6.31%-13.30% and 0.92%-4.35% respectively. 3) The overall release trends, transfer rates and the proportions of nicotine and glycerol in ACM increased with the rising of heating temperature. The nicotine content in the tobacco material was the key factor influencing nicotine release, and filter absorption was the key factor influencing glycerol release. The release of moisture increased with the rising of heating temperature, the proportion of moisture in ACM was higher than that of nicotine or glycerol. 4) There existed clear correlations among the releases of nicotine, glycerol and moisture, wherein the correlation between the releases of nicotine and glycerol was the closest.