Abstract: In order to investigate potential differences in the releases of aroma components from tobacco leaves of different types, planting areas and stalk positions under heating, a tobacco heating device with internal heating was developed and its matched tobacco rods were prepared. A total of 32 aroma components in the aerosols of 4 heated tobacco rods were analyzed by GC-MS using 55 mL puffing volume, 30 s puffing frequency, 2 s puffing duration, 10 puffs per tobacco rod. The releases of aroma components, including aldehydes, ketones, furans, pyrans, nitrogen-containing compounds, lower fatty acids, and higher fatty acids, etc., were determined. The samples of flue-cured tobacco, oriental tobacco and burley tobacco from different stalk positions and different planting areas were compared. The results showed that: 1) The developed quantitative method was suitable for analyzing the releases of aroma components with acceptable repeatability. 2) The releases of aroma components in aerosol of the heated tobacco of different types and from different planting areas and different stalk positions were clarified. The difference among different tobacco types was significant, and that among different planting areas was to a certain degree, while that among different stalk positions was slight.