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基于高通量测序的烟草粉螟基因组初步研究

Genome survey of Ephestia elutella based on high-throughput sequencing

  • 摘要: 为研究烟草粉螟基因组信息,基于低深度高通量测序,利用Illumina测序平台对烟草粉螟基因组进行了分析,采用K-mer法预测烟草粉螟基因组大小、杂合度和重复序列等基因组特征,并使用SOAP de novo软件对烟草粉螟基因组进行了初步组装。研究表明:①烟草粉螟预估基因组大小为546.4 Mb,杂合度为1.93%,重复序列比例为48.59%,GC含量为36.9%,属于复杂昆虫基因组。②基因组初步组装后,得到3 192 823条Contigs,Contig N50为244 bp,组装质量较低。对烟草粉螟基因组的初步研究为后续获得高质量烟草粉螟全基因组信息提供了必要参考。

     

    Abstract: In order to obtain the whole genome of Ephestia elutella, a genome survey of E. elutella was performed by low-depth high-throughput sequencing based on the Illumina sequencing platform. The genomic characteristics of E. elutella, such as genome size, heterozygosity and repetitive sequences were predicted by K-mer method and the preliminary genome assembly was conducted by SOAP de novo software. The results showed that the estimated genome size, heterozygosity, repetitive sequence ratio and GC content of E. elutella genome were 546.4 Mb, 1.93%, 48.59% and 36.9% respectively, which suggested that E. elutella genome was a complex insect genome. Additionally, 3 192 823 contigs were obtained after preliminary assembly and the contig N50 was 244 bp, which indicated the low quality of genome assembly. The genome survey provided an important reference for subsequent acquisition of high-quality genome information of E. elutella.

     

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