Abstract: In order to study the synergistic effects of fulvic acid and microbial agents on tobacco bacterial wilt and bacterial community in rhizosphere soil, a randomized block experiment was conducted to investigate the incidence of tobacco bacterial wilt in fields. The V4 region of bacterial 16S rRNA gene in rhizosphere soil samples was amplified and high-throughput sequencing was performed to analyze the bacterial community structures of all the treatments. The results showed that: 1) One hundred and twenty days after transplanting, the incidence and disease index of tobacco bacterial wilt significantly reduced by the synergistic treatment of fulvic acid and microbial agents. 2) Observed species, Shannon index and Simpson index of the bacterial community significantly increased by the synergistic treatment of fulvic acid and microbial agents. 3) The synergistic treatment of fulvic acid and microbial agents improved the bacterial community structure of rhizosphere soil, and significantly increased the relative abundances of Arthrobacter, Stenotrophomonas and Bryobacter. Meanwhile, the relative abundances of unidentified Burkholderiaceae, Streptomyces, unidentified Gemmatimonadaceae and Dyella significantly reduced. The synergy of fulvic acid and microbial agents had a good control effect on tobacco bacterial wilt, the diversity and structure of bacterial community in rhizosphere soil increased significantly.