Abstract: In order to clarify the effects of different fertilization measures on the soil respiration(autotrophic respiration and heterotrophic respiration) in southern Anhui tobacco growing areas, field experiments with three fertilization treatments of chemical fertilizer(CK), chemical fertilizer + bioorganic fertilizer(T1)andchemical fertilizer + high carbon basal fertilizer(T2)were carried out to evaluate the dynamic changes of soil respiration, temperature, moisture content and active organic carbon content. The results showed that: 1)The total soil respiration rate for all the treatments ranged from 0.99 to 7.39 μmol·m^-2·s^-1, and presented a trend of increasing first and then decreasing during the tobacco growing period with a peak appearing about 90 days after transplanting. The interaction of soil temperature and moisture content could explain 65.6%-75.3% of the variation of soil respiration rate. 2) Compared with CK, T1 and T2 treatments significantly increased soil respiration rates by 33.17% and 15.14%; wherein T1 significantly increased soil autotrophic and heterotrophic respiration rates by 34.66% and 32.08%, respectively. 3) The application of organic fertilizer significantly increased the contents of labile organic carbon, medium and high active organic carbon in soil. 4)Total soil respiration rate positively correlated with soil temperature, labile organic carbon, medium and high active organic carbon contents, while negatively correlated with soil moisture content. Therefore, the combined application of bioorganic fertilizer and high carbon base fertilizer could increase total soil respiration rate and promoted the accumulation of soil active organic carbon in tobacco fields