Abstract: In order to solve the challenge that strong polar analyte protectants are not well performed in weak polar solvent systems, the solution of acetonitrile: dichloromethane=2:1 (V/V) was chosen as weak polarity solvent system. The effects of 23 compounds as analyte protectants on the heights and shapes of chromatographic peaks of 31 aroma components were investigated. The chromatographic peaks of the aroma components in solvent added with these analyte protectants were compared with those in tobacco matrix. After optimizing the type, composition and concentration of the protectants, a suitable combination of analyte protectants was developed. In addition, 15 aroma components in tobacco (other than the 31 aroma components mentioned above) were chosen to verify the matrix compensation effect of the developed analyte protectant combination. The results showed that: 1) The suitable analyte protectants combination was malic acid (1 000 mg/L) + 1, 2-tetradecanediol (1 000 mg/L). 2) By adding the optimized analyte protectants combination into the solvent standard solution, a satisfactory matrix compensation effect was achieved. The linearity of the calibration curve for each aroma component was greatly improved, and the slope and linear correlation coefficient (r²) were basically consistent with the matrix-matched calibration curve. 3) The matrix effect of aroma components in tobacco during GC/MS analysis was compensated by adding analyte protectants.