Abstract: In order to study the effects of planting density and nitrogen application rate on cigarette smoke quality of flue-cured tobacco, a field split plot experiment at three levels and two factors was conducted to investigate the smoke characteristics of tobacco leaves. The results showed that: 1)Planting density significantly affected the total particulate matter, moisture content, tar release and the puffing number of the experimental cigarettes. At the same nitrogen application rate, the total particulate matter and tar release of the cigarette smoke decreased with the increase of planting density from 1 100 to 1 345 plants/667 m², especially for leaves of middle and lower positions. 2) Nitrogen application rates significantly affected the moisture content and tar release of the cigarette smoke, the tar release from leaves of upper and middle positions decreased with the reduction of nitrogen application rate. 3)The analysis of the interaction between the planting density and the nitrogen application rate indicated that the contribution to smoke quality by planting density was higher than that of nitrogen application rate, and there was a significant interaction between the two factors.