Abstract: In order to identify the members of inflorescence meristem receptor-like kinase (IMK) gene family which thought to be involved in tobacco inflorescence development and stress resistance, six IMK gene family members were identified in tobacco genome using bioinformatics methods, and the similarity of amino acid sequences among the different members were between 61% and 97%. Phylogenetic tree analysis showed that tobacco IMKs could mainly be divided into two types, and the number of repeating motif 12 (GSIP motif) was the major difference between the two types. Transmembrane domain analysis indicated that there were differences in the distribution of transmembrane domains between the two types of tobacco IMKs, suggesting that there might be functional differentiation between them. The results of post-translational modification site analysis showed that only two of the 11 modification sites identified in Arabidopsis IMK2 were conserved in all IMK proteins, suggesting that these two sites might play important roles in the function of IMK proteins. Gene expression analysis showed that the IMKs were highly expressed in lateral roots and flower buds, and were up-regulated by GA, IAA and other hormones, suggesting that IMK proteins might be involved in the development of lateral roots and flowers and participate in GA and IAA mediated regulatory responses.