Abstract: To investigate the effects of flue-curing and redrying on fungal community structure of tobacco leaves, tobacco leaf samples were collected from Meitan, Pingba and Huishui in Guizhou Province. The fungal community structure of the samples at different processing stages (before flue-curing, after flue-curing, before redrying and after redrying) was analyzed by high-throughput sequencing technology. A total of 4 phyla, 23 classes, 71 orders, 196 families, 445 genera, 724 species and 1 171 OTUs were detected from 12 leaf samples. The results showed that there were significant differences in the composition of tobacco fungi before and after flue-curing or redrying: at the genus level, Sampaiozyma and Symmetrospora were the dominant genera before flue-curing, and their relative abundances were 44.27% and 21.24% respectively. After flue-curing, Sampaiozyma, Alternaria and unclassified Didymellaceae were the dominant genera with relative abundances of 37.87%, 18.42% and 12.89% respectively. Before redrying, Aspergillus, Sampaiozyma and Rhizopus were the dominant genera with relative abundances of 60.82%, 24.12% and 2.51% respectively; after redrying, Sampiozyma and Aspergillus were the dominant genera with relative abundances of 46.12% and 25.78% respectively. Sampaiozyma were the dominant fungi at all four processing stages.