Abstract: To investigate the transfer behaviors of pesticides during puffing of heated tobacco products, standard solutions of six pesticides were added into the blank heated tobacco product samples. The mainstream aerosols of the prepared heated tobacco products were collected under the Canadian intensive puffing regime. The transfer rates in mainstream aerosols, filter retention rates and residues in the used tobacco section of the six pesticides were determined by gas chromatography-tandem mass spectrometry after purification with matrix dispersion solid phase extraction method. The results showed that: 1) The spiked recoveries of the six pesticides in the various substrates ranged from 75.5% to 117.4%, and the relative standard deviations were between 1.4% and 9.1%. 2) The transfer rates of the six pesticides in mainstream aerosols were mostly lower than 10%, and the addition levels of pesticides had no obvious effects on the transfer rates. 3) The filter retention rates of the residues were between 12.9% and 35.5%, and the residues in tobacco section were 41.9%-81.5% of the addition levels of pesticides.