Abstract: In order to identify the pyrolysis behaviors of tobacco leaves more efficiently and accurately, a method based on macro thermogravimetric analysis (MTGA) for identifying pyrolysis characteristics of cut tobacco was proposed. On the basis of differential thermogravimetry (DTG) results, tobacco leaves of different grades from different stalk positions and regions harvested in different years were analyzed and quantitatively characterized with root mean square error (RMSE). The differences identified by the MTGA were compared with those by thermogravimetric analysis (TGA), and the application of the MTGA in cigarette blending maintenance was verified. The results showed that under the condition of ensuring high efficiency and closer to the real situation of cigarette smoking, MTGA had the same identification ability as that of TGA, and the quality of substituted cigarette blends obtained via the MTGA was close to that of the original formula, and the style of the substituted blends were basically same as that of the original ones.