Abstract: To clarify the effects of plant growth promoting rhizobacteria (PGPR) on growth promotion and disease resistance of flue-cured tobacco, potential roles of previously isolated Bacillus velezensis F10 were studied through pot and plot experiments to investigate its growth promotion and control effects on tobacco bacterial wilt. The results showed that the growth of roots and aerial parts of tobacco plants under the biocontrol bacterium F10 treatment were significantly better than that of the control, and the activities of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) in tobacco leaves increased. The control effects of biocontrol bacterium F10 on tobacco bacterial wilt in pot and plot experiments were 60.49% and 54.91% respectively. The agronomic traits of flue-cured tobacco in fields treated with biocontrol bacterium F10 were significantly better than those of the control. The yield, output value, average price, proportion of high grade leaves, and proportion of high-to-mid grade leaves increased by 11.82%, 31.46% and 13.17% respectively. Biocontrol bacterium F10 has growth promoting effect on flue-cured tobacco and has good control effect against tobacco bacterial wilt, which significantly promotes the economic benefits of flue-cured tobacco.