Abstract: For testing ventilation rate, a reusable control sample was designed for cigarette test station. The control sample was composed of a wrapper layer of elastic material and a filler layer of porous material. The critical flow tubes used for draw resistance and ventilation rate testing were adopted to simulate the draw resistance and ventilation rate of cigarette. A quality control chart was used to monitor the testing process and effectively identify the problems in the testing process. The performance and applicability of the control sample were assessed and the effects in major application scenarios were analyzed. The results showed that: 1) The simulative testing of all cigarette physical indexes, including weight, circumference, draw resistance, hardness, length and ventilation rate, were realized. 2) The consistency and uniformity of the control sample was significantly better than those of cigarette. The coefficient of variation of all indexes was obviously lower than that of cigarette, that of hardness was lower than 2% and that of the other indexes was lower than 1%. The variation range of draw resistance was 9 Pa and that of ventilation rate was 1.7% during five successive years, and the means and standard deviations of the other indexes did not change basically. It suggested that designed control sample might be adaptable to cigarette test stations of different types. 3) The control sample performed very well in inter-laboratory collaborative testing, testing process monitoring, the repeatability and reproducibility evaluation of standard measurement methods and measurement auditing, it guaranteed the effectiveness and comparability of test results.