Abstract: In order to assess the application of eugenol-β-D-glucoside in cigarettes, synthesized eugenol-β-D-glucoside was analyzed by a thermogravimetric analyzer coupled with GC-MS (Py-GC-MS). The transfer and sensory characteristics of eugenol-β-D-glucoside in cigarette smoke were also investigated by GC-MS. The results showed that: 1)The thermal stability of eugenol-β-D-glucoside was significantly improved compared with eugenol. 2)Eugenol-β-D-glucoside was pyrolyzed initially at about 220 ℃. The main pyrolytic product was eugenol, with the other products including furfural, phenol, 2-methoxyphenol, etc. 3)Eugenol was detected in the mainstream smoke of the cigarettes with added eugenol-β-D-glucoside, and its release increased with the increase of glycoside addition rate. The eugenol featured good aroma release stability and consistency by this method. Different flavoring effects could be achieved via different cigarette flavoring methods. In conclusion, eugenol-β-D-glucoside could be used as a stable flavor precursor in cigarette flavoring.