Abstract: To reduce the starch contents (mass fraction) in cured tobacco leaves and regulate the synthesis of resistant starch in tobacco leaves, the leaves of tobacco cv. Yunyan 87 were analyzed for the variations of resistant starch contents, the correlation between resistant starch contents and amylose contents, and the gene expression levels related to starch synthesis in the tobacco leaves. The results showed that the contents of total starch and resistant starch in the tobacco leaves presented an increasing trend generally during the growth of tobacco, in which the middle to late growth stage was the main accumulation period of resistant starch. There was a significant positive correlation between the resistant starch content and amylose content (r=0.992**, P < 0.01). In the curing process, yellowing stage was the main degradation stage of resistant starch and non-resistant starch in the tobacco leaves, and the degradation proportion of resistant starch was lower. There was a significant positive correlation between resistant starch content and amylose content during curing (r=0.918*, P < 0.05). During the growth and development of tobacco, the expression levels of starch synthesis-related genes AGPS2, AGPS3, AGPL, SS2, SBE1, ISA3, GBSS and SBE2 were basically stable or increased continuously; among them, the variation trends for GBSS and SBE2 were consistent with the variation trends of amylose content and resistant starch content. GBSS1 and SBE2 might be the key genes regulating amylose and resistant starch contents.