Abstract: In order to accurately measure the puff-by-puff cut tobacco consumption during cigarette smoking, a device for real-time monitoring cigarette mass loss during puffing and smouldering was designed based on the principle of moment balance. Combined with puffing-to-smouldering ratio experiments, the puff-by-puff cut tobacco consumption during smoking was obtained. The influence of filter ventilation rate on the puff-by-puff cut tobacco consumption and the relationship between the air intake from the combustion cone and cut tobacco consumption were investigated. The results showed that: 1) The designed device could accurately measure the mass change of a burning cigarette with the precision RSD less than 1%. 2) The cigarette smoldering and puffing processes could be clearly distinguished via the mass loss curve of the cigarette, and the mass loss of each puffing was calculated according to the cigarette mass difference before and after puffing. The puff-by-puff mass loss of a burning cigarette increased with the proceeding of puffing. 3) Combining with the puffing-tosmouldering ratio experiments, the puff-by-puff cut tobacco consumption of the cigarette was obtained, and the cut tobacco consumption of experimental cigarette samples 1#, 2# and 3# ranged from 19.9 to 26.2 mg during smoking steadily. 4) The cut tobacco consumption of cigarettes at the same puff number decreased with the increase of filter ventilation rate. 5) There was a linear relationship between air intake from the combustion cone and cut tobacco consumption, and the cut tobacco consumption per milliliter air intake from the combustion cone correlated to the combustibility of cut tobacco.