Abstract: In order to evaluate the safety of four new herbicides applied in preceeding rice fields to succeeding tobacco, the effects of the four herbicides on tobacco growth were investigated through indoor pot experiments and field plot experiments. A QuEChERS pretreatment method combined with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) were employed to measure the residual level of quintrione in tobacco field soils. The results showed that florpyrauxifen, triafamone·tefuryltrione and mesotrione had no significant effects on the growth of tobacco. The inhibition rates of quintrione (750-2 250 g a.i./hm²) to the leaf length, leaf width and plant height of tobacco in the pot and field experiments were 48.27%-69.00%, 66.30%-78.08%, 58.76%-75.83% and 24.89%-43.79%, 35.47%-49.81%, 28.87%-48.75%, respectively. Quintrione caused serious phytotoxicity to succeeding tobacco. No quintrione (the residue level was lower to the LOQ, 0.002 mg/kg) was detected in the soils when tobacco seedlings were transplanted in the following year. Thus, there is a great risk that quintrione would cause phytotoxicity to succeeding tobacco, and it should be used with caution in tobacco-rice rotation areas.