Abstract: In order to investigate the effects of organic acids on releasing behavior of nicotine in heated tobacco products with randomly cut tobacco filling, GC-MS analysis method was used to analyze the influences of adding different mass fractions of citric acid, tartaric acid, malic acid, levulinic acid, lactic acid, propionic acid and acetic acid on the transfer rate of total nicotine, the pH of aerosol and the forms of nicotine in the aerosol of a heated tobacco product. The results showed that: 1) The addition of these organic acids did not affect the transfer rate of total nicotine significantly. 2) The pH of aerosol also did not change significantly after adding the non-volatile organic acids; however it decreased slightly and continuously with the increase of volatile organic acids. 3) The proportion of free nicotine in the aerosol was not significantly influenced by the addition of non-volatile organic acids, while it decreased obviously and continuously with the addition of volatile organic acids.