Abstract: In order to clarify research status, research hotspots, development courses and future trends in the field of drought stress in tobacco, VOSviewer and CiteSpace were used for visual analysis after data mining of the documents on drought stress in the core database of Web of Science from 2002 to 2021. The results showed that: 1) The number of publications on drought stress in tobacco had increased, with China in a leading position in the amount of literature. 2) The literature from the United States, Japan, and Spain had more influence, and there was less international cooperation in the published literature. 3) The main lines of research on drought stress in tobacco were transgenic tobacco breeding, application of plant growth regulators, drought domestication and screening of drought tolerant varieties. 4) With the development of life science and global warming, genetic engineering, research and development of efficient plant growth regulators, stress of multifactorial factors, new type of omics and combined multi-omics analysis might become the research hotspots in the future.