Abstract: In order to reveal the puff-by-puff release behavior of ester and alcoholic monomer flavors from filter tow of demi-slim cigarettes, the transfer rules of representative esters and alcoholic monomer flavors during smoking of demi-slim cigarettes were studied using ultrasonic assisted extraction combined with gas chromatography (GC) method. The results showed that: 1) The puff-by-puff transfer rates of 17 esters and 13 alcoholic monomer flavors were in the range of 0.31%-20.43% and 1.54%-19.60%, respectively, with those of acetic esters and straight chain saturated alcohols being greater and those of cinnamates and unsaturated alcohols having relatively stable puff-by-puff releases. 2) For the same puff number, the puff-by-puff transfer rates of each group of homologous monomers of ester and alcoholic monomer flavors increased with increasing boiling points, and those of each group of isomeric monomer flavors increased with increasing molecular structural stability. 3) Puff-by-puff transfer rates of ester and alcoholic monomer flavors increased with proceeding puff, and there was a good linear positive correlation between the cumulative puff-by-puff transfer rates and puff numbers.