Abstract: To investigate the potential risk of chemical components released from the wicking cotton of e-cigarettes, the risk assessment of wicking cotton was conducted. The chemical components including volatile, semi-volatile, and non-volatile compounds and metallic elements generated during the working process of e-cigarette were screened by non-targeted, two-dimensional gas chromatography-tandem quadrupole time-of-flight high-resolution mass spectrometry (GC × GC-QTOF-MS), ultra-performance liquid chromatography-quadrupole time-of-flight high-resolution mass spectrometry(UPLC-QTOF-MS), and inductively coupled plasma mass spectrometry(ICP-MS), respectively. A risk assessment of the detected chemical components was performed. The results showed that: 1)A total of 72 chemical components belonging to eight classes were detected in two different samples, most of which were volatile chemical components with mass fractions below 3 mg/kg. 2)All detected chemical components were screened for SCT(Safety Concern Threshold)and QT(Qualification Threshold)values, with nine identified organic components and the metal elements Ti likely to be of high exposure and potential risk for the two products. Further comparison with relevant data reported by the US FDA and other agencies indicated that the results for the four chemical components, including furfuryl alcohol, furfural, diethanolamine and Ti, with the highest inhalation exposure were well below the health guidance values. The results of the risk assessment showed that the exposure levels for the screened chemical components were all well below the health guidance values and did not raise safety concerns.