Abstract: In order to promote the puff volume accuracy and aerosol trapping efficiency in the puff-by-puff aerosol analysis of new type tobacco products, an array type multi-port puff-by-puff smoking machine was designed. The smoking machine mainly consists of a puffing module, a trap feeding module, and a holding and sealing module. The motor speed and piston stroke in the puffing module are variable to achieve different puffing profiles and puff volumes. Simultaneous multi-port puffing improves the analysis efficiency. Sequential control technology is used to switch the trap and collect the aerosol puff-by-puff. The two ends of the trap are connected in parallel to the puff volume calibration pipeline and in conjunction with the holding and sealing module to ensure no puff volume deviation during puffing. Repeatability of the puff volume of the smoking machine under single-puff and puff-by-puff conditions was tested with iQOS device and"Marlboro"Heatsticks. The results showed that: 1)The pipeline of the smoking machine had good air tightness, and the calibrated puff volume was accurate. The puff-by-puff release trend of each component from the heatstick was consistent with that reported in literatures. 2)The RSD of total release of each component from the heatstick was below 4% and the RSD of puff-by-puff release of each component was below 5%, it meant good repeatability and stability. 3)The puff-by-puff trapping efficiency of each component from the heatstick was higher than 96%. This technique provides support for the rapid and accurate analysis of the aerosol components from new type tobacco products on a puff-by-puff basis.