Abstract: To study the sensory-related amide components in cigarette smoke, 21 amide compounds in mainstream cigarette smoke were determined by gas chromatography-tandem mass spectrometry combined with dispersive solid phase extraction. The analytical method for amides was developed after optimization of key pre-treatment conditions and instrumental parameters, such as extraction, purification, chromatographic separation and multi-reaction monitoring of mass spectrometry. Twelve commercial cigarette samples of different brands with different tar yields were tested for their releases of the 21 amides in mainstream smoke and the influencing factors. The results showed that: 1) Graphitized carbon black was selected as the adsorbent for dispersive solid phase extraction due to its better purification effect for cigarette smoke matrix and higher recoveries for the 21 amides, and the established analytical method had higher sensitivity, better accuracy, and stronger anti-interference ability. 2) Due to their higher boiling points, the amides were mainly distributed in the particulate matter of mainstream smoke. 3) Among them, sixteen amides were detected in cigarette samples with a release range of 0.4-303.9 ng/mg (normalized to average release per mass unit TPM), and the remaining five amides were not detected. 4) The releases of 12 amides were highly positively correlated with TPM, with the correlation coefficient being higher than 0.8. 5) T-test results indicated that the releases (normalized to per mass unit TPM) of 7 amides from blended type of cigarettes were significantly higher than those from Virginia type cigarettes.