Abstract: In order to study the influences of cigarette design parameters, smoking parameters, and burn rate on the oxygen consumption of a lit cigarette under machine-smoking condition, a method for testing oxygen consumption of a burning cigarette was established by combining a smoking machine with paramagnetic oxygen analysis. The variations of oxygen consumption during smoking with different cigarette design parameters under different smoking conditions were investigated. The relationships between the oxygen consumption, the burn rate of cigarette, and the routine chemical components in mainstream cigarette smoke were studied. The results showed that: 1) The oxygen consumption of a burning cigarette increased with the increases of puffing volume, puffing duration, expanded cut tobacco content, reconstituted tobacco content, the grammage, potassium sodium ratio, and filler content of cigarette paper and cigarette circumference; while it decreased with the increases of tobacco weight, expanded stem content and the air permeability of cigarette paper, it also increased first and then decreased with the increases of cut tobacco width and the burn improver content in the cigarette paper. 2) The oxygen consumption of a burning cigarette significantly positively correlated to the cigarette's burn rate during puffing, the smouldering rates, and significantly negatively correlated to the contents of nicotine, TPM and tar in the mainstream smoke, and puffing number, did not significantly correlate to the contents of moisture and CO in the mainstream smoke.