Abstract: To investigate the effects of glycerol on the isothermal thermogravimetric behavior of cut tobacco strips and the thermal release characteristics of key components in heated tobacco products, a macro-thermogravimetric analyzer was used to study the isothermal thermogravimetric behavior of cut strips with varying glycerol contents at 200 ℃, and the thermal release characteristics of glycerol, nicotine, moisture, and other components during the weight loss process were analyzed. The results showed a significant effect of glycerol addition on the isothermal thermogravimetric behavior of the cut strips. The presence of glycerol resulted in noticeable changes in the weight loss curve, indicating a shift in the thermal degradation process. Glycerol also affected the release characteristics of the key components. Increasing the glycerol content within the experimental range of 0-13.35% resulted in higher weight loss ratio and weight loss rate of the cut strips, longer preheating time of the cut strips, and decreased stability of the component release process. The weight loss ratio of the cut strips increased from 18.14% to 23.79%. The proportion of nicotine release increased from 95.62% to 96.49% with a higher release rate. However, both the release proportions and the release rates of glycerol, moisture and other components decreased. Specifically, the glycerol release proportion decreased from 88.69% to 80.20%, moisture from 82.13% to 64.03%, and other components from 15.82% to 12.63%. The thermal release rates of glycerol, nicotine and other components in the cut strips first increased and then decreased, while moisture showed an opposite trend.