Abstract: In order to reveal the main factors influencing the color of tobacco for cigar wrapper, conventional air-curing and phased sampling methods were used to study the changes of moisture content, chlorophyll content (mass fraction), enzyme activities, chemical composition and colorimetric values of cigar tobacco at different air-curing humidity. The results showed that the moisture loss rate of cigar tobacco leaves in the process of air-curing under low humidity treatment was higher than that under medium humidity treatment, and that of high humidity treatment was the lowest. Under high humidity treatment, the degradation of chlorophyll a and chlorophyll b, and the increasing rates of carotenoid in cigar tobacco leaves were faster. The activities of peroxidase and polyphenol oxidase in cigar tobacco leaves under high humidity treatment were higher than those under low humidity and medium humidity treatments, especially at the later stage of air-curing (20-30 d). The colorimetric values of cigar tobacco leaves after air-curing were the highest under high humidity treatment, followed by medium humidity treatment and low humidity treatment. Correlation and stepwise regression analyses indicated that the correlation coefficients between the reducing sugar, total nitrogen and colorimetric values reached extremely significant (P≤0.01) levels, and reducing sugar and total nitrogen were the important factors affecting the color of tobacco for cigar wrapper after air-curing.