Abstract: To understand the effects of phyllosphere microbial communities on tobacco leaves infected with angular leaf spot, the composition and diversity of phyllosphere microbial communities on diseased and healthy tobacco leaves were analyzed using high-throughput sequencing technology. The results showed that the phyllosphere bacteria of diseased and healthy tobacco leaves were mainly Firmicutes, Actinobacteria, and Proteobacteria, and the fungi were mainly Ascomycota at the phylum level. For diseased leaves, the dominant genera of bacteria were Pseudomonas, Methylobacterium, Xanthomonas, Stenotrophomonas, Sphingomonas, Pantoea, and unidentified_Rhizobiaceae, and those of fungi were Plectosphaerella, Didymella, Gibberella, and Alternaria. For healthy leaves, the dominant genera of bacteria were Pseudomonas, Sphingomonas, Pantoea, and Methylobacterium, and those of fungi were Plectosphaerella, Didymella, Cladosporium, and Boeremia. Richness and diversity indices of phyllosphere bacterial and fungal communities of diseased tobacco leaves were higher than those of healthy tobacco leaves. Functional predictions showed that the metabolic pathway functions of the bacteria in both diseased and healthy leaves were mainly metabolism, environmental information processing, and genetic information processing. The functional floras of fungi in diseased leaves were mainly plant pathogen, plant pathogen-undefined saprotroph, animal pathogen-endophyte-plant pathogen-wood saprotroph, and endophyte-plant pathogen. The functional floras of fungi in healthy leaves were mainly plant pathogen, plant pathogen-undefined saprotroph, endophyte-plant pathogen, and undefined saprotroph. Community composition, diversity, dominant genera and relative abundance of phyllosphere bacteria and fungi in diseased and healthy leaves differed to varying degrees.