Abstract: To investigate the spraying effects of different plant protection sprayers on tobacco and their control efficacy on tobacco brown spot disease, the atomization performances, operating efficiencies and control effects by electric knapsack sprayers combined with different nozzles and a plant protection UAV spraying at different flying heights were evaluated in tobacco fields. The results showed that the electric knapsack sprayer combined with multi-link fishbone 8 nozzles had the highest drug liquid adhesion rate on tobacco, and the adhesion rate was 68.33%. The drug liquid was evenly distributed on the upper, middle and lower leaves of tobacco plants, and the consumption of the drug liquid was reduced by 33.23% as compared with the electric knapsack sprayer combined with conical hollow double nozzles. The absolute control efficacy was 18.52% as compared with the electric knapsack sprayer combined with conical hollow single nozzles, and the spraying effect was the best at the moderate gear. The electric knapsack sprayer combined with the multi-link fishbone 8 nozzles and the plant protection UAV spraying at a flying height of 2.0 m were used to spray the drug liquid for tobacco from fast growing stage to mature stage, and the droplet density distribution uniformity and coverage distribution uniformity of the liquid on tobacco leaves were the best with the average droplet particle size ranged from 125.00 to 148.30 μm, which were in accordance with the best biological particle size range of pesticide spraying to prevent and control tobacco diseases. The control effect on tobacco brown spot was better with the relative control effect of 33.26% and 29.36%, respectively, and the operating efficiency was 1.20 and 36.24 times higher than that of electric knapsack sprayer combined with conical hollow double nozzles, respectively.