Abstract: In order to investigate the differences in the mass fractions of organic acids in smoke of different types of reconstituted tobacco materials, a quantitative method using a tube furnace to generate smoke that could be captured for analysis was developed. Silylation derivatization combined with GC-MS/MS was used to determine the captured organic acids in smoke under different conditions. A comparative study was conducted on the mass fractions of organic acids in different types of flue-cured tobacco and reconstituted tobacco made from them. The results showed that: 1) The detection limits of 29 organic acids were between 0.83-17.57 ng/g, and the spiked recoveries ranged from 85.68% to 108.32%, with RSD in the range of 1.19%-7.66%. The method was suitable for the quantitative determination of the organic acids in tobacco. 2) The total amounts of the organic acids in both flue-cured and reconstituted tobacco materials were detected by this method at different temperatures and environmental atmospheres, and it was found that the total amount of the organic acids in reconstituted tobacco was greater than that in the corresponding flue-cured tobacco. An aerobic atmosphere was conducive to the release of organic acids, and the maximum amounts of the organic acids occurred when the heating temperature was 250-300 ℃.