Abstract: In order to grasp the current development status and trends in the field of tobacco biotechnology, the incoPat database was used to search the tobacco biotechnology patents in China from 2000 to 2022, and the obtained patents were analyzed in terms of application trend, IPC subclass distribution, and patent technology fields. The results showed that: 1) The field of tobacco biotechnology had entered a period of rapid development, with an average of 140 patent applications per year. 2) Domestic tobacco industries had obvious advantages in the number of patents in this field, with Yunnan Provincial Tobacco Company having the largest number of patents, followed by China Tobacco Yunnan Industrial Corporation Limited, Zhengzhou Tobacco Research Institute of CNTC and Guizhou Provincial Tobacco Company. Overseas applications were mainly from multinational tobacco companies, among which the United States Smokeless Tobacco Company and Altria Client Service Company had the largest number of applications. 3) The distribution of IPC technical topics in this field was extremely concentrated, mainly involving C12N (microorganism and its medium) and C12Q (method of determination), and A01N-A01P-C12R (biological bacterial agents for disease resistance, insecticidal or plant growth regulation) and A01H-C07K-C12N (cultivation of new plant varieties or tissue culture) were the focus of patent layout in the future. 4) In terms of specific technologies, it had mainly focused on the fields of green prevention and control of tobacco plants, mechanism of action of tobacco genes and tobacco biological bacterial agents, especially the green prevention and control that involved tobacco virus detection and biological control of pests and diseases.