Abstract: To characterize the structure and function of NtMYB102 tobacco transcription factor, the CDS of NtMYB102 gene was cloned from tobacco cultivar K326 by homology-based cloning and Sanger sequencing. Physicochemical characteristics, domains of NtMYB102 protein and upstream sequence of NtMYB102 gene were analyzed by online tools. The expression level of NtMYB102 gene after Ralstonia solanacearum infection was analyzed based on transcriptom raw data downloaded from NCBI SRA. Relative expression level of NtMYB102 gene after being infested by aphids was analyzed by qRT-PCR. The results showed that: 1) The NtMYB102 gene was 1 131 bp in length and encoded a protein consisting of 376 amino acids. The NtMYB102 protein had typical MYB domains, and was a hydrophilic protein with phosphorylation sites while lacking signal peptides and transmembrane structure. 2) The 2 000 bp sequence upstream of the NtMYB102 gene contained several regulatory elements associated with stress and pathogen response. 3) At 36 h after infection by R. solanacearum, the relative expression level of the NtMYB102 gene of Yanyan 97 (resistant to tobacco bacterial wilt) was significantly lower than that at 0 h, suggesting that this gene may be involved in coping with infection by R. solanacearum. 4) The relative expression level of the NtMYB102 gene of Zhongyan 100 was significantly increased after aphid infestation, indicating that this gene may be involved in the response of tobacco to aphids.