Abstract: To obtain entomopathogenic fungi with high pathogenicity to Myzus persicae, a fungal strain was isolated from the naturally infected M. persicae and designated as HFLD1. The genus and species of HFLD1 were identified by morphological and molecular biological methods. The pathogenicity of HFLD1 against the wingless adult of M. persicae was investigated by a laboratory dip method. The field control efficacy of HFLD1 against M. persicae was tested by the spraying method. The results showed that HFLD1 was Lecanicillium dimorphum. The laboratory experimental results showed that aphid mortality increased with increasing concentration of HFLD1 conidial suspension. After inoculation with an 1×10⁸/mL concentration of HFLD1 conidial suspension for seven days, the corrected cumulative mortality of M. persicae reached 95.06%, and the median lethal time(LT₅₀)was 3.28 days. The results of the field trial indicated that the control efficacy of HFLD1(1×10⁸ conidia/mL) on M. persicae reached 79.87% at seven days after the treatment. Therefore, HFLD1 had strong pathogenicity and good control efficacy against M. persicae, and the fungal strain could be a potential resource used for biological control of M. persicae.