Abstract: To investigate the key factors influencing the moisture reabsorption characteristics of reconstituted tobacco, the effects of temperature, relative humidity and storage time on the moisture reabsorption characteristics of reconstituted tobacco sheets and finished cut reconstituted tobacco from drying, rolling and slurry process were investigated using response surface methodology. By analyzing the morphological characteristics and the number of functional groups of reconstituted tobacco, a correlation between the physicochemical structure and the moisture reabsorption characteristics of reconstituted tobacco was discovered. The results showed that: 1) The effect of temperature on the moisture reabsorption characteristics of tobacco was slight, while the effect of relative humidity was significant. 2) Reconstituted tobacco from the drying process had a high initial moisture content due to its porous structure from multi-layer stacking, which easily absorbed or retained moisture, while reconstituted tobacco from the rolling or slurry process and finished cut reconstituted tobacco had a smoother surface morphology resulting in a lower initial moisture content. 3) The larger the number of hydroxyl functional groups in the reconstituted tobacco, the smaller the increase in moisture reabsorption, while the larger the pore volume of the reconstituted tobacco, the larger the increase in moisture reabsorption. Therefore, the preparation technology for the reconstituted tobacco could be optimized based on these findings.