Abstract: To determine the appropriate values of cut tobacco parameters for discrete element simulation, the physical repose angle of cut tobacco was measured by physical tests. The optimal values of cut tobacco simulation parameters determined by the Plackett-Burman test, the steepest ascent search test and the Box-Behnken test were used in a virtual cut tobacco stacking experiment. The results showed that: 1) The physical repose angle of brand A cut tobacco was 28.59°. 2) The parameters that significantly affected the repose angle of brand A cut tobacco were the coefficient (X₄) of tobacco-tobacco static friction, the coefficient (X₅) of tobacco-tobacco rolling friction, the coefficient (X₇) of tobacco-stainless steel static friction, and the optimum values of these three coefficients were 0.589, 0.123 and 0.422, respectively. 3) The simulation experiment was carried out using the optimal combination of the parameters. The relative error between the virtual and physical repose angle of brands A, B and C was less than 5%, and the coefficient of variation of the virtual repose angle was less than 1%. The calibration method had good repeatability and the simulation parameters were considered to be accurate.