Abstract: In order to screen highly effective agents suitable for the control of tobacco target spot by plant protection unmanned aerial vehicle(UAVs), 8% jinggangmycin SL was mixed with 4 chemical agents (25% pyraclostrobin SC, 430 g/L tebuconazole SC, 40% fludioxonil SC, 30% difenoconazole propiconazole EC), and through indoor toxicity test, pot control test, field spray test and field control effect test, the compounded agents suitable for the control of tobacco target spot by unmanned aerial vehicle were screened. The results of the indoor toxicity test showed that when jinggangmycin was mixed with pyraclostrobin, tebuconazole and difenoconazole propiconazole at the mass ratios of 7 ∶ 3, 9 ∶ 1 and 2 ∶ 1, the co-toxicity coefficients were 353.392 1, 200.831 7 and 197.350 7, respectively, indicating their synergistic effects. The results of the pot control test showed that the relative control efficacy of the mixture of jinggangmycin and pyraclostrobin at the mass ratio of 7 ∶ 3 was 67.46%, significantly (P < 0.05)higher than that of the other treatments. The results of the field spray test showed that the density, deposition and coverage of droplets on the middle part of tobacco plants treated with the mixture of jinggangmycin and pyraclostrobin at the mass ratio of 7 ∶ 3 were 50.96%, 69.65% and 82.02% were higher than those treated with jinggangmycin. The results of the field control effect test showed that the relative control efficacy of the mixture of jinggangmycin and pyraclostrobin at the mass ratio of 7 ∶ 3 and that of jinggangmycin and tebuconazole at the mass ratio of 9 ∶ 1 were 61.83% and 60.46%, respectively, significantly (P < 0.05) higher than that(50.46%)of jinggangmycin treatment. In conclusion, the mixture of jinggangmycin and pyraclostrobin at the mass ratio of 7 ∶ 3 is more suitable for the control of tobacco target spot by plant protection UAVs.