Abstract: To investigate the differences in sensory quality and the key aroma components in tobacco leaves from different tobacco planting areas in Bijie, 42 samples of C3F flue-cured tobacco leaves were collected from 8 tobacco planting counties (districts or cities) in Bijie. The sensory quality of the samples was evaluated and the aroma components in the samples were analyzed by headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS). The results showed that: 1) There were clear differences in sensory quality of tobacco leaves from the different areas, and the quality of tobacco leaves from the central and western areas was generally higher than the eastern area. 2) A total of 318 volatile components were identified, in which 13 aroma components with VIP (Variable projection value) > 1 and rOAV (Relative odor activity value) > 1 were screened by using OPLS-DA model analysis and rOAV analysis. Furthermore, the key aroma components contributing to the leaf style difference from the different areas in Bijie were identified through the correlation analysis of the sensory quality indexes, they included β-damascenone, geranyl acetone, cis-3-hexenyl valerate, 2,5-dimethyl-4-hydroxy-3(2H)-furanone, 2-phenylethanol, and phenylacetaldehyde. 3) The relative contents of 2-phenylethanol and 2,5-dimethyl-4-hydroxy-3(2H)-furanone in tobacco leaves from the different areas in Bijie were higher, which contributed to the honey and sweetness aroma notes which were prominent in Bijie tobacco leaves.