Abstract: In order to clarify the main nutrients and their contents in Myzus persicae nymphs, the contents of carbohydrate, protein, amino acid, vitamin, cholesterol, fat, fatty acid and elements in Myzus persicae nymphs were investigated. The results showed that the total sugar content in Myzus persicae nymphs was (4 551.273 1 ± 6.219 7) mg/100 g, and 7 different sugars were detected the highest glucose content was (2 213.972 5 ± 2.397 2) mg/100 g. The crude protein content was (11.973 2 ± 0.453 1) g/100 g. Seventeen free amino acids were detected (the highest content of alanine was 0.206 2 g/100 g) and eighteen hydrolyzed amino acids were identified (the highest content of glutamate was 0.687 6 g/100 g). Among the ten essential amino acids, lysine had the highest content, with hydrolyzed lysine and free lysine measured at 0.098 1 g/100 g and 0.661 5 g/100 g, respectively. The vitamin C content was 463.497 0 μg/100 g, with inositol having the highest content among the water-soluble B vitamins at 9 277.739 4 μg/100 g. The crude fat and cholesterol contents were (11.521 7 ± 0.158 2) mg/g and (1.720 0 ± 0.004 1) mg/g, respectively. Sixteen types of unsaturated fatty acids were detected (the highest content of linoleic acid was 246.258 1 mg/100 g) and fourteen types of saturated fatty acids were identified (the highest content of myristic acid was 2 223.241 1 mg/100 g). Among the 49 elements tested, potassium, iron, magnesium, zinc, sodium, manganese, sulfur, copper, phosphorus, molybdenum, and tin had relatively higher contents, and potassium, iron, magnesium, zinc, and sodium all exceeded 10 mg/kg. The results laid the foundation for the development of artificial diet for natural enemies with Myzus persicae as prey.