Abstract: Four plant fiber-based papers(softwood, hardwood, cotton and fluff fibers)and reconstituted tobacco were studied to investigate the fiber types suitable for the production of reconstituted tobacco by the papermaking-slurry composite process. The physical properties and morphological structures of these fibers were analyzed, and their thermal transition performance and the release of key constituents (moisture, glycerol and nicotine) from reconstituted tobacco in aerosols were compared. The results showed that: 1) The fluff fiber-based paper exhibited excellent liquid absorption capabilities with an absorption height of 86.6 mm, indicating superior loading effects for coating liquids. The softwood fiber-based paper exhibited superior mechanical properties with a tensile index of 63.47 N·m/g, enabling it to successfully withstand coating pressure and ensure consistent coating quality. 2) The reconstituted tobacco made from fluff fibers had a dense internal structure, making it an ideal carrier for flavoring agents. The softwood fiber reconstituted tobacco had a more porous internal structure, which facilitated the release of aerosol constituents. 3)Within the temperature range of 50-125 ℃, the reconstituted tobacco made from softwood fibers exhibited a lower weight loss rate, minimizing the diffusion and loss of key constituents. Within the temperature range of 150-400 ℃, the temperature corresponding to the peak weight loss rate of the dry base of softwood fiber reconstituted tobacco was relatively lower, ensuring the complete and stable release of aerosol constituents. 4)The softwood fiber reconstituted tobacco initially contained relatively lower levels of moisture, glycerol and nicotine, but exhibited higher release rates of the three key constituents under the same heating conditions, indicating better overall performance in terms of key constituent release.