Abstract: Accurate quantification of carbon emissions is essential to developing a green low-carbon logistics system. To establish a well-developed, systematic and accurate method for quantifying the carbon footprint of cigarette road transport by tobacco manufacturing enterprises and to quantify transport-related carbon emissions, this work considered a typical cigarette road transport scenario involving diesel-powered freight vehicles and applied an improved emission-factor method to quantify carbon emissions per case. Key influencing factors were selected, and an empirical model was developed to assess the influence of different variables on carbon emission per case (10,000 cigarettes). The results showed that transport distance was significantly and positively associated with carbon emission per case, and a 1% increase of transport distance was associated with an approximately 0.810% increase in carbon emissions per case. Vehicle load factor was significantly and negatively associated with carbon emission per case, and a 1% increase in load factor was associated with an approximately 1.123% decrease in carbon emissions per case. The findings provide a theoretical basis and practical support for managing carbon emissions from cigarette road transport in tobacco industry.