Abstract: In order to achieve rapid determination and quality assessment of polyphenols in tobacco and tobacco products, a quantitative analysis of multi-components by single marker (QAMS) method was established for the simultaneous determination of eight polyphenolic compounds, including neochlorogenic acid, scopolamine, chlorogenic acid, cryptochlorogenic acid, caffeic acid, scopoletin, rutin and kaempferol 3-rutinoside. The analysis was performed using high-performance liquid chromatography with diode array detection (HPLC-DAD) for a segmented detection program. Separation of the eight polyphenols was achieved on a C18 column using a mobile phase consisting of methanol, water, and acetic acid under a gradient elution mode. The flow rate was set at 1.0 mL/min, and the column temperature was maintained at 30 ℃. Using chlorogenic acid as the internal reference substance, the relative correction factors for the other seven polyphenols were calculated. The mass fractions of the eight components in tobacco and tobacco products were determined and compared using both the external standard method (ESM) and the QAMS method (calibrated via multi-point, slope, and single-point methods). The results showed that: 1) Under the optimized conditions, the eight polyphenols exhibited good linear relationships within their respective concentration ranges (R2＞0.999 9), and the limits of detection (LODs) and quantification (LOQs) were 0.01‒0.15 and 0.02‒0.48 mg/L. The spiked recoveries of this method ranged from 92.00% to 102.38% with relative standard deviations (RSDs) of 1.56%‒4.10%. 2) Using chlorogenic acid as the internal reference, the relative correction factors calculated by the slope or multi-point correction method demonstrated good durability and reproducibility (most of RSDs < 3.00%). Furthermore, the slope correction method achieved the highest consistency with the external standard method, yielding an average relative deviation of no more than 3.21%. 3) The established method is simple, accurate, and suitable for the batch determination and quality evaluation of eight polyphenols in tobacco and tobacco products.