Abstract: To research the response of tobacco to excitation, the experiments on tobacco cultivars, G140, Hongda, NC89, G28, K326, and Xujin2, were conducted with Harpin_Ea as the exciton. 30μg/mL aqueous Harpin_Ea solution was injected into tobacco leaves to excite their hypersensitive response, then the soluble proteins in tobacco before and after excitation were analyzed by SDS-PAGE and scanning analysed with a gel imaging analysis system. The results showed that the contents of soluble proteins in all treated tobacco cultivars were changed, some increased significantly and some reduced, besides, there were new soluble proteins composed in some cultivars.