全二维气相色谱/飞行时间质谱法分析烟草挥发和半挥发性酸性成分

Analysis of Volatile, Semi-volatile Acidic Components in Tobacco with Comprehensive Two-dimensional Gas Chromatograpy/Time-of-flight Mass Spectrometry

摘要: 采用全二维气相色谱/飞行时间质谱(GC×GC-TOFMS)法和GC-TOFMS定性分析了烟草中挥发性、半挥发性酸性物质。结果表明,在相同条件下,GC-TOFMS只鉴定出烟叶中的有机酸24种,酚类4种,而GC×GC-TOFMS经自动数据处理,结合结构谱图辅助定性和手动纠错,最终定性出挥发性、半挥发性酸性成分共有107种,其中有机酸有82种,酚类25种。

Abstract: The volatile and semi-volatile acidic components in tobacco were analyzed qualitatively by comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry (GC×GC/TOFMS) and GC/TOFMS.The results showed that only 24 organic acids and 4 phenols were identified by GC/TOFMS;however, a total of 107 acidic components, including 82 organic acids and 25 phenols, were characterized by GC×GC/TOFMS via automated data processing combined with chromatogram aided qualitative analysis and manual rectification.