反相HPLC法同时测定烟草中的6种有机酸

Simultaneous Determination of Six Organic Acids in Tobacco with Reverse-phase High Performance Liquid Chromatography

摘要: 建立了用反相高效液相色谱法同时快速测定烟草中柠檬酸、苹果酸和草酸等6种有机酸的方法。采用的色谱柱为Hypersil C18(250mm×4.6mm,5μm),流动相0.01mol/L NaH₂PO₄(pH2.98)和无水甲醇(体积比为98:2),流速为1.0mL/min,柱温25℃,检测波长210nm。有机酸的回收率为98.2%~104.3%;相对标准偏差为0.61%~3.38%;检出限0.04~0.53μg/mL,分析时间不超过7min。并分析了4种转光地膜和普通地膜覆盖的K326初烤烟叶中这6种有机酸的含量。

Abstract: A method for simultaneous and rapid determination of 6 organic acids (including citric, malic and oxalic acid) in tobacco with reverse-phase high performance liquid chromatography (HPLC) was developed.HPLC was performed on a Hypersil C18 column (250mm×4.6mm, 5μm) at 25℃, using 2% CH₃OH-98%NaH₂PO₄(0.01mol/L;pH2.98) as mobile phase at the flow rate of 1.0mL/min and the detection wavelength of 210nm.The recoveries of the organic acids ranged from 98.2%to 104.3%with relative standard deviations of 0.61%-3.38%, and the limits of detection were 0.04-0.53μg/mL (S/N=3).The runtime was less than 7min.The 6 organic acids in cured tobacco leaves of K326 covered with four sun light-conversion films and one ordinary film were also analyzed.