Abstract: The effects of combining ability and heritability of seven yield and quality-related numerical characters (including plant height, leaf number, the length and width of middle leaf, yield, output value and the proportion of high grade leaves) of six burley tobacco varieties were analyzed.The results showed that the general combining ability (GCA) between parents and specific combining ability (SCA) between cross combinations reached extremely significant or significant level, which indicated that these characters were controlled by both additive effect and non-additive effect genes, and some characters were dominated by the additive effect gene, and others were dominated by both.There were relatively wider differences between GCA values of different characters from the same parents, the SCA values of crosses and the GCA values of parents were not directly correlated.