HPLC法测定特制卷烟及烟气中的前胡甲素、岩白菜素和槲皮素

HPLC Determination of Praeruptorin A, Bergenin and Quercetin in Purpose-made Cigarette and its Smoke

摘要: 为了解中药有效成分向烟气中的转移率,采用高效液相色谱法测定了添加前胡、矮地茶、槐米提取物的卷烟烟丝及其烟气中这些提取物中的有效成分——前胡甲素、岩白菜素和槲皮素的含量,采用的色谱柱为C₁₈ TURNER Kromasil TM(3.0mm×250mm,5μm),流动相分别为甲醇:水(75:25,体积比)、甲醇:水(20:80)、甲醇:0.4%磷酸(42:58),流速均为1mL/min;检测波长分别为321、275和360nm。结果表明,前胡甲素和岩白菜素向烟气中的转移率分别为1.01%~1.77%和0.41%~0.48%,烟气中未检测到槲皮素。

Abstract: For investigating the rate of active substances in traditional Chinese medicines transferring into cigarette smoke, the active substances, praeruptorin A, bergenin and quercetin, in test cigarettes incorporated with extracts of Radix peucedani, Ardisia japonica, Sophofae japonicae and in their smoke were determined by HPLC with TURNER KromasilTM-C₁₈ column (3.0mm×250mm, 5μm), using methanol:H₂O (75:25, v/v), methanol:H₂O (20:80), methanol:0.4% phosphoric acid (75:25) as mobile phase (flow rate 1mL/min) at the wavelength of 321nm, 275nm, and 360nm, respectively. The results showed that the transfer rates of praeruptorin A and bergenin into cigarette smoke were 1.01%-1.77% and 0.41%-0.48%, respectively; no quercetin was detected in the smoke.