Abstract: The moisture transport and dissipation in and from the upper leaf of flue-cured tobacco harvested together with 6cm long stalk (leaf at the top, middle and bottom of stalk respectively) were studied, the results showed that during flue-curing, the moisture content in all parts of leaf prominently decreased with the advance of curing time; some of the moisture in stalk lost because of high temperature and some was transported to vein and lamina, and the transport of moisture from stalk to vein and then to base, middle and tip parts of leaf was unobstructed. According to the whole moisture dissipation and transport trend of leaf, the curing quality of leaf at the bottom of stalk was better than that at the top or middle of stalk.