Abstract: The contents of volatile bases in 170 flue-cured tobacco samples grown in 6 provinces of China in 2002 and 2003 were determined.The results showed that:1) the contents of volatile bases in these samples were in the range of 0.10% to 0.45%;2) for the same grade, the volatile bases content in tobacco grown in Mile County of Yunnan Province was the highest, that in Bijie County of Guizhou Province and Huili County of Sichuan Province was higher, that in Yongding, Changting and Longyan Counties of Fujian Province and Linyi County in Shandong Province was lower, and that in Yiyang County of Henan Province was the lowest;the contents of volatile bases in tobacco grown in different areas differed significantly;3) for the same growing area, the volatile bases content in lower leaf was the lowest, that in upper leaf was the highest and about 2 times the concentration of that in lower leaf;and the difference was extremely significant;4) for the same growing area and same stalk position, the content of volatile bases in orange leaves was higher than that in lemon leaves, and the difference was extremely significant;5) the volatile bases content in tobacco leaf increased gradually with its body from thin to thick, oil from poor to rich and color from light to dark, and the difference was extremely significant.